Forces on cells and pressure ulcers, a force microscopy study.
Pressure ulcers (PU) constitute a major and costly health issue, one particular difficulty is the early detection of PU formation and our group is developing a novel pressure sensor system to tackle this issue. Another challenge is to understand the cellular mechanisms of PU formation. In this project individual cells relevant to PU formation (muscle, skin, etc.) will be mechanically loaded using an atomic force microscope (AFM) and their response will be analysed by standard fluorescence microscopy techniques to detect eventual damage such as membrane rupture, cytoskeleton damage, etc..The possible release of cytokines will also be examined. Such basic research study would help develop a better understanding of PU formation, essential for improving PU treatment and care amongst patients. The work involves extensive use of AFM microscopy, fluorescence microscopy and biological cell preparation techniques. It provides opportunities for inspired students with background in physics, material science, biomedical, or mechanical engineering or chemistry
Personnel Involved
First Supervisor: Lemoine, P Dr
Second Supervisor: Burke, G Dr
Collaboration: This project does not involve collaboration with another establishment